qPCR Gene Expression Signature

Gene Expression Signature in Peripheral Blood as a Marker of Parkinson’s Disease

Abstract/Summary

Parkinson’s disease (PD) is the second most common neurodegenerative disorder, characterized by the progressive depletion of dopaminergic neurons within the substantia nigra. The disorder clinically manifests as progressive symptoms of tremors, rigidity, bradykinesia and postural instability. PD usually occurs late in life – its incidence increases with age and has a prevalence of approximately 1.8% among people over the age of 65, translating to significant effects on health, morbidity and mortality in the overall population. Diagnosis of PD represents an ongoing challenge, as lengthy diagnostic procedures are required to establish certain diagnosis. For this reason, there is great interest in developing biomarker‐based methods for the early and non‐invasive detection of PD.

In this application, NGS libraries were prepared from E. Coli DH10B genomic. Five genes were selected based on the results of past global gene expression studies, and a qPCR validation study was performed on samples taken from an independent subset of patients with PD and matched controls. The qPCR experiment was designed and prepared using the PIPETMAX® qPCR Assistant software and the PIPETMAX 268 automated pipetting instrument.

Figure_2_qPCR-curves_parkinsons

Representative amplification plot produced during qPCR run. A wide range of amplification of Marker gene 4 can be seen across 15 patients (red) and 16 control (blue) samples.


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