QPCR

Gene Expression Plant – Virus Study Monitoring Potato Virus Y (PVY) with Real-Time PCR (qPCR) Results Using the PIPETMAX® 268

Abstract/Summary

Gene expression studies are often used to gain insight into complex interactions between organisms. Real‐time PCR (qPCR) is frequently used in gene expression studies as it fits perfectly with its wide dynamic range, sensitivity, and ease of automation possibilities.

In this application the PIPETMAX 268 was used for automated sample preparation in advance of qPCR which was performed on the sampled leaves from both virus-inoculated and mock‐inoculated potatoes. The discussion describes the results for the relative expression of the Chlorophyll a/b binding gene (CAB) and PVY RNA relative gene expression levels, linear regression values from serial dilutions, cytochrome oxidase (COX) gene amplification curves, and statistical estimation of pipetting (%CV).

 

COX-Gene-Amplification-Curve-PIPETMAX
COX Gene Amplification Curves of Standard 5‐Fold Dilution Curve. Amplification Curves From the Left to Right are of the Following Dilutions: 5x, 25x, 125x, 625x, and 3125x.


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