Next Gen Sequencing (NGS)

Automated NGS Library Prep: Ligation Efficiency of NxSeq® Sample Preparation Using the Gilson PIPETMAX® 268

Abstract/Summary

As the number of samples for sequencing increases, there is a need for library prep automation that matches the throughput of the Next Generation Sequencing (NGS) instruments while not requiring an investment equal to the sequencer itself. By utilizing the Gilson PIPETMAX® 268, a novel NGS sample prep chemistry was automated to reduce the time to achieve the final prepared library, increasing the efficiency of the method and consistency of the resulting DNA library to be sequenced.

In this application, NGS libraries were prepared from E. Coli DH10B genomic DNA (gDNA) and FAM-labeled adaptors using the NxSeq® DNA Sample Prep kit, comparing the manual method with an automated library prep protocol on the PIPETMAX. This application note describes the results of the ligation efficiency of manual library preparation versus automated library preparation.

 

COX-Gene-Amplification-Curve-PIPETMAX
Comparison of Manual NxSeq® Libraries (light blue, pink) and PIPETMAX NxSeq Libraries (red, green) using a 0.8:0.2 ratio of AMPure XP Beads for size selection.


To See the NGS Data:

Download Application Note